I can extract side effects.

Chapter 627 The Mystery of Frequent Miscarriages of Sows
"The genetic modification was a success!"

Seeing the picture of the cells under the microscope, Huang Qin breathed a sigh of relief, and finally let go of his hanging heart.

There was a burst of cheers behind him.

"Yeah, I just said it's fine."

"That's it?"

"It's great, long live the big brother."

"Don't be too happy, this is just the first step."

"Yes, the next cloned embryo is the key."

The students talked in a hurry, each of them couldn't hide their excitement.

Afterwards, they turned their heads in unison, looking expectantly at their respected elder brother.

"When is the next step?"

Huang Qin looked at the expectant eyes of his juniors and sisters, and couldn't help laughing.

I think back when I forced them to do experiments every day, these rookies all showed resistance, as if life would be worse than death.

If it weren't for the carrot of graduation hanging in front of him, he would doubt whether these people could persevere.

I didn't expect it, but now I'm gearing up and I can't wait.

And in turn urged himself to come.

With this situation, he couldn't help but think of the time when his first experiment was successful and the first project had results.

I am also so excited that I wish I could spend 28 hours a day in the laboratory, witnessing miracles of nature one after another.

Thinking of this, Huang Qin couldn't help raising the corners of his mouth, and comforted him with a smile.

"I know you are in a hurry, but don't worry, we will start right away."

"You have mastered the basic process of cloning mice by nuclear transfer."

The students nodded one after another, obediently like chickens pecking at rice.

"Okay, I'll give a demonstration first, and then you can divide the cells to make pig nuclear transfer clones."

"When the time comes, I'll see who has the best embryos."

The students immediately burst into smiles.

"Oh, big brother, I've cloned mice a hundred and eighty times. I'm absolutely familiar with it. I won't let you down."

"Okay, look at mine! Let me show you what it means that blue comes from blue and is better than blue."

"Well done, is there any reward?"

Huang Qin touched his head and smiled.

"Hey, do you still want the green to be better than the blue? It seems that I underestimated you."

"Okay, as long as everyone's embryo can survive for more than 20 days, I will take you to the group building for two days. You can choose the place, how about it?"

In general, during the process of cloning animals, there are three peaks of embryonic death.

About 10 days is the first peak period. In the first 5 days, the pig's egg cells will divide into 70-100 cells, forming a structure called blastocyst.

Until about 10 days, the blastocyst will hatch into an embryo, ready for implantation in the uterus.

During this period, the environment of the uterus must be imitated in vitro, and a slight mistake can easily cause the death of the embryo, with a mortality rate as high as 20%.

About 20 days is the second peak period. Embryos have been implanted, but their organs have not yet formed. They are all competing for the nutrients secreted by the placenta. Some embryos with weaker abilities will lose in the competition and die one after another.

Embryo mortality at this stage is as high as 15%.

About 60 days, will usher in the third peak period.

But this stage is okay, as long as adequate nutrition is provided, it can be avoided and the mortality rate will be greatly reduced.

So in the whole cloning process, the first 20 days are very critical.

Not only must they develop into embryos in vitro, but they must also be successfully implanted into sows and survive the period of competition between them in order to survive.

This is also the reason why Huang Qin proposed the assessment.

After hearing this, the students all showed embarrassment. After all, the embryo of a pig is more complicated than that of a mouse. The successful cloning of a mouse does not mean that a pig can be successfully cloned.

Besides, this is a batch of genetically modified pigs, who knows what kind of results a genetic modification will cause.

It is very likely that the entire army will be wiped out, and none of them will survive.

The tall and thin boy gritted his teeth and said loudly, "No problem, I have confidence in myself."

Huang Qin immediately cast an admiring look. He had noticed this tall and thin boy a long time ago. He is excellent in every aspect and is a good seed for scientific research.

Seeing that someone came forward, others also joined in.

"Look at mine, senior brother, I will definitely give you a big surprise."

"For these two days of team building, I will fight no matter what!"

"Wow, it's winter right now, and I want to go to the warm beach to bask in the sun."

"If you don't want to go to the beach, it's also a good thing to find a hot spring hotel, soak in the hot spring for two days, eat and drink."

Huang Qin nodded in satisfaction. Since the papers were almost finished, the pressure on graduation dropped sharply.

For the next difficult work, he can only rely on money to motivate everyone, and now it seems that the effect is very good.

The students stayed in the ivory tower for a long time, and they were easily bribed before they saw the horror of sugar-coated shells in the outside world.

"It's still college students who are so foolish! This is going to be replaced by the old fritters in our department, and it's not so easy to be fooled."

He couldn't help sighing in his heart: "University students are so easy to use. It would be great if the teacher could bring a few more students."

The next thing is easy.

Huang Qin first demonstrated it to everyone.

The sows used in the laboratory are quite different from ordinary domestic pigs. They are specially optimized miniature pigs.

It is about the size of a dog, and weighs only 15 kilograms even when it is mature.

The piglets used in the experiment are all 3-5 month old piglets, which weigh only a few kilograms like cats, and are extraordinarily small and exquisite.

They have fluffy white body hair, black flaky patterns on the back, pink pig nose and mouth, and rolling eyes, which are very cute.

But no matter how cute it is, it must dedicate itself to human technology.

"Eldest brother, let me catch second senior brother, hehehe!"

The female classmate with a high ponytail volunteered to stand up, smiling happily.

She took a gilt out of the cage. This pig had been injected with chorionic gonadotropin several times in a row, and it was induced into a state of high egg production.

Looking at the pink and cute little pig in her hand, the female student showed a look of unbearable expression, but her movements were unambiguous.

With the needle raised and lowered, a tube of anesthetic was injected, and the gilt instantly fell into a coma.

Fresh oocytes were then collected from the oviducts of sows.

Under the microscope, Huang Qin carefully removed the nucleus of the oocyte with a thin tube with a diameter of about 7 microns, and removed the cytoplasm as much as possible.

While doing it, he explained the precautions to his classmates, and he didn't forget to make a few jokes.

"After taking out the cell nucleus, it needs to reciprocate several times in the glass tube, so that the small amount of cytoplasm carried can be removed."

"Using the same method, the genetically modified pig cells should be injected directly into oocytes that have had their nuclei removed within 5 minutes after the nucleus has been removed."

"The movements must be fast, firm and precise, without making mistakes or procrastinating."

"It will definitely be a bit of a rush at the beginning, but when you do it more times, practice will make perfect."

"The cloning experiment is done well. Even if you don't do scientific research in the future, it will be of great use to do test-tube babies."

"Of course, even if the country works hard to give birth, I don't want you to do it all at once. Hahaha~"

"Okay, after the nuclear transfer is complete, put the oocyte in this special solution and let it stand for 1-6 hours."

"When the situation is right, divalent strontium ions (Sr2+) and cytokinin B are added for treatment."

"The former activates the oocyte, and the latter inhibits the formation of polar bodies and the exclusion of chromosomes."

"The specific situation that is suitable for the law depends on my brother's experience."

"Let me tell you, it can't be judged at a glance without soaking it in the laboratory for three to five years..."

"After the treatment is complete, the oocytes are taken out and placed in a special solution without strontium and cytostatin B."

"This is the moment to witness the miracle. The oocyte will gradually divide, form a blastocyst, and slowly become an embryo."

Huang Qin fully presented the whole process in front of the students, and at the same time shared his own experience without hesitation.

Under his Chunchun guidance, within a week, everyone learned how to handle pig oocytes.

There were several failures during the period, but fortunately, there were enough genetically modified cells in the laboratory, which supported everyone to complete the cloning experiment.

The development of oocytes is also very smooth, and they all develop into embryos of different stages.

Subsequently, embryos at different stages were implanted into sows in a pseudopregnant state for subsequent development.

Embryos that develop earlier, such as those at the cell stage and blastocyst stage, are implanted in the sow's oviduct, where implantation occurs naturally.

Those that develop to the blastocyst stage are directly implanted into the uterus, skipping this step.

Each of the students was assigned a sow, and after completing a series of treatments, they began to observe quietly.

Everyone is waiting for the critical threshold of 20 days to come.

They were in mixed moods, both uneasy and full of confidence.

The smooth progress of the previous experiments gave them an illusion.

It seems that this cloning experiment is not very difficult.

As long as the movements are proficient, it is not easy to do well.

As for the sows to conceive and give birth, there is nothing difficult about it.

Haven't seen the pig run, haven't you eaten pork yet?
As a tenacious animal like a pig, there will definitely be no problem.

However, things in scientific research are not always so satisfactory.

"Oops, my baby Zhu Bao has no fetal movement, what's going on?"

In the laboratory, the female student with the high ponytail suddenly let out a scream, and the whole person became panicked.

She leaped onto the cage, looked at the bleeding sow below her, and let out a burst of exclamation.

However, her cry did not attract any response from the students.

Even if the other students heard it, they were shocked, and immediately devoted all their attention to their own sow, how could it be possible to care about her affairs.

The tall, thin boy next to him stared intently at the sow in the cage in front of him, completely immersed in the care of the sow.

The whole person seemed to be deaf, and the screams from a classmate one meter away did not affect him in the slightest.

The screams seemed to be contagious, and after a while, the fat boy on the other end also let out a scream.

"Ah! My sow has miscarried!"

"Your sister, the first time I delivered a baby, I dedicated it to a sow!"

The fat boy's cry was even louder, and it sounded unusually penetrating.

At this moment, his chubby face was flushed red, his expression was surprised, shocked, and even a hint of disbelief.

The whole person froze at the scene, looking at a loss.

However, no matter how unacceptable he was, he had to face the bloody fact.

After reacting, I quickly dug out the electronic version of "Postpartum Care of Sows" and quickly browsed it on my mobile phone.

"Well, don't panic, find a solution first."

"Here, after a sow has a miscarriage, it is first necessary to judge whether the stillbirth has been completely drained."

"If it is not completely excreted, the sow should be injected with cloprostenol first, so that the cervix of the sow can be opened to expel the stillborn fetus, otherwise the stillborn fetus will rot in the uterus, making the sow infertile."

"After the row is clean, inject Guanlin Pioneer + Zengjian instant food needle to reduce inflammation in sows for 3 consecutive days, which has a good preventive effect on sow inflammation."

"Huh~~ It's okay, it's okay, luckily I've been prepared."

After finding a solution, the fat boy let out a long breath and calmed down.

According to the method described in the book, he carried out meticulous post-abortion treatment on the aborted sows, and finally saved the precious lives of the sows.

However, he didn't waste the stillbirth that flowed out, so he quickly took it under a microscope to observe it to find out what caused the miscarriage.

The same tragedies happened one after another in the laboratory.

The horrific screams came one after another, one after another, and even spread outside the laboratory.

On this day, all the teachers and students passing by Du Biao's laboratory were whispering to each other.

"Hey, you passed Teacher Du's laboratory today, did you hear any strange noises?"

"Ah, I was just about to tell you, my God, I don't know what happened inside, howling ghosts and wolves, scared the students upstairs enough, thinking that some human tragedy or experimental accident happened."

"I'll go. I'm just downstairs from them. There are ghosts screaming upstairs all day, which makes it impossible for me to do experiments."

"Isn't it a ghost hit?"

"Don't talk nonsense, talk about science. I think poisoning is more likely."

"No, I didn't see the ambulance coming either?"

"You said, could they be infected with swine fever or something?"

"It's possible. In the past few weeks, I have seen people in their lab, everyone is red-eyed, and they walk listlessly. They must be sick."

"Everyone, please stay away, don't get infected."

"I sneaked a few glances through the glass window. The people inside kept screaming heart-piercingly. It felt like a walking dead. Oh my god, it's really scary."

The news spread rapidly on the campus, and soon reached the ears of the hospital.

So, in the distant capital, Du Biao, who had just attended an academic conference, received an urgent call.

"Teacher Du, something happened in your lab."

"What? Something went wrong? What's going on?"

Du Biao was at a loss. Two days ago, he received a call from a student, reporting that he was doing a pig cloning experiment and everything was going well.

"Dean Lu asked you to come back quickly and deal with it. All of your students are not in the right state, and the school does not dare to interfere too much."

"Understood." Du Biao could tell that the matter was serious. The students must have gone wrong with the experiment. They were under too much psychological pressure and were on the verge of collapse.

Immediately bought a plane ticket, hurried back.

He made preparations for the aftermath, and also made plans to suspend all meetings and give psychological counseling to each of the students.

However, he never expected that when he pushed open the door of the laboratory.

What appeared in front of him turned out to be such a scene.

(End of this chapter)