I can extract side effects.

Chapter 626 100 Gene Edited Pigs

Suzhou University, Sanqing Branch.

"Hehe, Xiao Du, you have become popular on the Internet recently."

Dean Lu swiped his phone leisurely, and said cheerfully to Du Biao who had just entered the door.

Du Biao had just arrived at the dean's office and hadn't started reporting work yet, so he couldn't help being stunned when he heard this sentence.

"What online?"

"It's a hot search on the Internet, you can see it on any platform, and the netizens have given you a famous nickname, what is it called...Professor Buffalo?"

Dean Lu elongated his tone, spit out these four words slowly, and immediately burst out laughing.

Du Biao's expression instantly became confused: "But I don't study buffaloes, I study pigs, how can I get it wrong."

Dean Lu shook his head with a smile: "You idiot, Buffalo doesn't mean that. It means that your water thesis level is very good, so I call you Professor Buffalo."

Du Biao's face flushed immediately: "Scientific research, can it be called water? That's called... that's called..."

In desperation, I thought about it for a long time, but couldn't say why.

Dean Lu looked at him with interest, then shook his head in disappointment: "Hey, I thought you could come up with something famous."

Du Biao angrily took out his mobile phone, casually swiped the Internet, and saw his name appearing on the hot search lists.

#水牛Professor highlights the chaos of scientific research irrigation
#生化环材 Becomes a Popular Irrigation Area
#Gusu University A professor published more than 40 papers in half a year, suspected of serious irrigation
Du Biao's face darkened, and his anger instantly filled up.

"These more than 40 papers are all students' papers. I just changed the manuscript and posted a corresponding author. It's not even a first paper. Can this be blamed on me? Are you not allowed to bring more students?"

"Besides, it's a good thing for students to be able to publish papers. A little water doesn't matter. It's better than studying for a few years and not being able to write papers. At least my students' experiments have been done steadily."

"This group of media really don't know fart, they know nonsense every day."

"Okay, okay, the courtyard didn't mean to blame you." Dean Lu waved his hand and comforted him casually.

"It's all a bunch of outsiders making a fuss. I've read a few of your student's papers, and they're pretty good. Some of them can be published in the second division of SCI, which is no longer considered water."

"The others are a little bit worse, but they are also papers with detailed data and exquisite experimental design. According to my standards, even if they are not so eye-catching, they are definitely not watery."

"Moreover, these are all horizontal projects of enterprises, and the money is all paid out of the three clearinghouses. It is pure nonsense to say that the state's scientific research funds are wasted."

"The main thing is that our college temple is small, and a big Buddha suddenly appeared, so it's normal to have some criticism."

"But the principal will be very happy when he finds out, and told me to let you bring more students next year."

"I heard that many students think it's easy for you to publish papers, and they want to apply for your graduate student. Tsk tsk!"

"Ah? I already have enough graduate students." Du Biao cried out with an aggrieved face, "I'm out on social every day, so I don't have time to take care of my students."

"Eight graduate students are not too many. You have two who just graduated this year. These two places have to be filled." Dean Lu said thoughtfully.

"As for the rest, let's wait until you get some grades."

Du Biao heaved a sigh of relief and nodded again and again: "No problem, I have finished some basic work for this project, and it is progressing as planned, and certain results will be produced soon."

When he said this, Dean Lu immediately became interested.

"Aren't you a horizontal project with Sanqing? Do some kind of xenotransplantation."

"This topic is very promising. There are very few domestic researches in this area. Overseas countries have done it for more than ten years, and there are some achievements. We have to catch up."

"Many people don't do innovative projects like xenogeneic organ transplantation, because they don't know when the results will come out. It may take five or ten years. Xiao Du, you young people are still aggressive."

"Don't worry, if you make some achievements in this project, Jieqing will be stable, and no one will compete with you."

"But what step have you made now? Let's hear it."

Du Biao was both surprised and delighted. He knew that Dean Lu was also an outstanding youth. Since he dared to say that, he must have a certain amount of confidence in him.

It seems that I made the right bet this time. With the shareholder style of innovation, maybe I can really get a good young man.

Although he seldom stays in the laboratory, he has carefully read the papers of the students and knows the situation of the laboratory well.

Immediately, he reported the progress of the project one by one, and Dean Lu nodded repeatedly.

After reporting to the dean, he walked on the school's boulevard.

Du Biao swiped his phone and saw the insults against him on Kuihu and Langbo, his face turned blue and white with anger, and his mood fluctuated with the comments.

"I'm speechless, why are all people who don't understand talking about it?"

"Well, people are afraid of being famous and pigs are afraid of being strong, especially in the Internet age, being famous is really not a good thing."

"Hey, some passers-by are praising me, saying that I am much better than those who pursue academics and finally force students to jump off buildings, those who drop out of school, and those who delay graduation. I deserve respect."

"Well said! Sure enough, there are still good people on the Internet, and the eyes of the masses are sharp."

When I couldn't stop swiping, two familiar colleagues suddenly walked up to me, both professors from the Department of Biology.

Seeing him from a distance, he started waving, and then showed a bright smile.

"Yo, isn't this our buffalo professor?"

"Old Du, now you are famous. Hahaha~"

"..." Du Biao's face froze, and he really wanted to turn around and leave, pretending he didn't see him.

But after thinking about it, he greeted him with a smile on his face.

It's just that I muttered silently in my heart.

"Forget it, the infamy will be borne by the teacher. As long as you can really research something, even if your reputation collapses and you are called Professor Buffalo, I will admit it."

******
In the laboratory, Huang Qin carefully sprinkled the prepared ARC1 solution on the cells in front of him, covered the petri dish, and gently placed it in a 37-degree constant temperature incubator.

Then he stood in front of the incubator and waited quietly.

Eight heads of various shapes poked out from behind him, staring intently at the cell solution inside through the glass door.

It can be seen that everyone's face is a little nervous, even Huang Qin can't hide the trace of uneasiness deep in his heart.

"The last step, just wait for two hours."

"It should be fine."

Huang Qin seemed to be soothing the restless emotions of the students, and at the same time cheering himself up.

The fat boy said worriedly: "Is it true that there will be no problems if a pig cell has been modified with so many genes?"

As soon as the words were out of his mouth, he was stared back by the stern eyes of his classmates, so he shrunk his neck and dared not speak again.

"Don't be crowed!" The female classmate with a high ponytail scolded, with clear eyes and confidence.

"Most of what we knocked out were endogenous retroviral genes, and no matter how many of these junk genes were removed, it would not affect the stability of the DNA itself."

"That's right, 65 PERV and other viral genes, we have removed all the viral genomes from the pig DNA."

The tall and thin boy's eyes flashed with fanaticism, and he blurted out.

"What's important are the other 36 gene sets, 29 immune-related genes, 2 coagulation-related genes, 4 kidney-related genes, and 1 insulin-related gene."

"This is a big project and a record, and it must not fail."

"We've made too many changes, won't the pig's genome completely collapse?"

Another male classmate muttered to himself, with a look of worry on his face.

"You don't care about it so much, just do it and finish it. There are so many edits in front, shouldn't it be good?"

A classmate cursed and spoke in a very impatient tone.

"Look at your face, it's all wrinkled into a mess. Look at it a little bit, failure is not success, damn it, if it doesn't work, just do it again, what else can you do."

Seeing that the results of the experiment have not yet come out, everyone quarreled first.

Huang Qin waved his hand, wanting everyone to be quiet.

As soon as the hands were raised, before they could speak, the students shut their mouths obediently as if they had been enchanted, as docile as quails.

"Stop making noise! ​​Quiet!"

He turned around, faced everyone, and said in a deep voice.

"Everyone, don't be impatient, we have reached the last step, just wait patiently."

"Whether it is successful or not, you will get the result in two hours."

The students nodded subconsciously, and the nervousness gradually dissipated, leaving only the expectation of the result.

To them, the plain words of the elder brother have a different kind of magic power. As long as he says it can be done, everyone will believe it without any doubt.

Over the past six months, Huang Qin's strength has completely conquered this group of students. His precepts and deeds, superb experimental skills, and generous money making ability have created an absolute authority among them.

Everyone admires him from the bottom of their hearts, loves him, worships him, and their eyes are shining like stars when they look at him.

Anything, as long as he says that he can do it, everyone feels that there is no problem. Even if there are setbacks, success will come sooner or later.

This kind of personality charm will unite everyone closely and form an unbreakable small team.

In their eyes, the mentor is just a loving father, and Huang Qin is the omnipotent elder brother, the absolute core of the team.

Seeing that the atmosphere became serious, Huang Qin smiled again.

His voice is like a bell, his eyebrows are kind and his eyes are kind, and there is a wonderful affinity in his smile. His words are firm and logical, which makes people have to believe.

"We have transformed the genomes of 101 pig cells this time, which is also an unprecedented feat in the history of xenotransplantation research."

"In the past, foreign research generally changed three or four genes, and the maximum number would not exceed eight."

"As for the virus genome carried by pigs, there is no problem. It must be knocked out, otherwise, after organ transplantation, it will lead to unpredictable infections in the human body. This has been verified in previous experiments."

"Exogenous viruses can be avoided by improving the environment. As long as the pig raising environment is clean enough, there will be no problem."

"The endogenous virus PERV can only be dealt with by gene knockout. We knocked out so many in order to make the safety as preventable and controllable as possible."

"The focus of past experiments has always been to solve acute and chronic rejection. Our current research basically solves the immune rejection of organs and tissues such as the heart, kidney, lung, and islets."

"But there are still some problems. For example, the liver, in addition to detoxification function, also has some metabolic functions, including the synthesis of blood coagulation factors, fat metabolism, etc."

"Although the liver of pigs can also synthesize these things, the products are not very suitable for humans. If the liver of pigs is directly used to provide these functions, the effect will be very poor."

"So the organ we transformed this time is mainly the simplest kidney. This time, we humanized 4 kidney-related genes to make the kidneys of pigs and humans more compatible, hoping to increase the survival rate of organs after transplantation."

"Except for the kidney, the islet cells of pigs are also very similar to the human body. The first generation of insulin used in clinical practice was extracted from pigs and cows, so it is called animal insulin."

"The composition of insulin in different animals is slightly different. Among them, the structure of pig insulin is the most similar to that of human, only one amino acid at the carboxyl end of the B chain is different, while bovine insulin has 3 amino acid differences, so pig islets are also very suitable for human transplantation. "

"We also engineered pig islet cells this time to produce human insulin."

"In addition, we have also modified a lot of immune genes. We have humanized almost all affected immune genes, which can prevent human antibodies from attacking pig organs."

"There are also two coagulation genes that prevent blood from clotting due to inflammation."

"In theory, such a transformation should reduce the rejection of pig organs to various body fluids to zero. At the same time, the genetic expression of kidney and islet cells also completely obeys that of human cells."

"Of course, there are too many modifications, and I don't know whether such pig embryos can mature."

When Huang Qin said this, he spread his hands and showed a helpless expression: "But isn't that what science is like?"

"Keep exploring, keep thinking boldly, then carefully seek proof, and keep doing experiments to verify."

"Even if we make mistakes, we can learn lessons and continue to improve. Even if we fail, we have gained experience. This wave is definitely not a loss."

"However," he changed his voice and continued, "I'm still very confident in my thinking."

"Next, let us wait and see."

Time is waiting, passing by every minute.

As Sanqing’s gene editing tools were later improved, the onset time of the arc1 protein was greatly accelerated.

In the past, it took at least 12-24 hours for gene editing to produce results. This time, using the latest arc1 protein, it only takes 2 hours to know whether the gene editing is successful.

Two hours later, Huang Qin led a group of students to the incubator again.

He took a deep breath, took out the petri dish, and looked at the transparent liquid inside.

I silently said "Bodhisattva bless, God bless, God bless...".

After begging all the gods and Buddhas in his heart, he finally observed the cells under the microscope.

(End of this chapter)