my life simulator

"Virtual gene chip!"

With an order, a light flashed, and a huge glass plate appeared in the laboratory.On this glass plate, countless arrays of gene probes are densely integrated.

This is a gene chip.

As long as there is a theoretical basis, the virtual laboratory can virtualize the finished product.

On the basis of gene chips, together with optical cables, consoles, computers, electron microscopes, monitors and other equipment, a test bench can be virtually combined.

After a while, various components were automatically matched to form a huge genetic testing test bed.

Then, Lu Li could conduct the gene expression test.

There are currently 30 genes that have confirmed the gene expression information of human beings, but... under the base of [-] billion, [-] is completely negligible.

Another advantage of the virtual laboratory is that any experimental material can be produced as long as one thinks about it.

So... Lu Li virtualized 30 billion cells that were missing a certain base pair.

Install these cells in the solution tank, start the genetic detection test bench, let these cells with missing base pairs flow into the gene chip continuously, cycle continuously, and compare continuously.

On the display screen, all kinds of data surged like a waterfall, and gene expression detection was being carried out continuously.

The solution in the solution tank flows through the gene chip continuously, and is continuously recycled, and the cycle runs without stopping.

It's so much easier!

Just wait for the test to finish and get the final result.

Lu Li let out a long breath, put aside the unimaginably large amount of repetitive labor of genetic testing, and began to study more interesting gene targeting technology.

At present, in gene editing technology, it is still a matter of luck how to accurately position and how to cut the shear protein to the desired position.

If it is possible to decipher all the genes in the human DNA chain and confirm the gene expression information of each base pair, then...it is very important to accurately cut the required gene fragments.

If you can hit the target at one time, you can hit every shot without missing the target, which will undoubtedly increase the efficiency of gene editing technology by tens of thousands of times.

In the existing gene editing technology, gene positioning depends entirely on transcriptase.

Transcriptase is like a radar.After the target gene is transcribed, the transcriptase obtains the information characteristics of the target, and then scans and searches on the gene chain to compare the target.

After finding the target, the transcriptase combined with the splicing protein will drive the splicing protein to attach to the gene chain and perform the splicing operation on the target gene.

In theory, this principle is completely correct.It's just that...the "radar" is relatively ineffective, and it often fails to find the target, or even finds the wrong target.

Now, what Lu Li needs to do is how to improve the radar's search capability.

But...it's hard to do!

Transcriptase is a very simple protein molecule, and there is no possibility of replacement on the "hardware device".

The hardware cannot be tampered with, and the only way is to update the software to improve the resolution.

To improve the resolution ability of transcriptase, it is necessary to master all the gene information on the gene chain.

Well, back to square one.

Or we can only do gene expression detection first, and first determine the gene expression information of 30 billion base pairs.

Engaging in genetic research depends entirely on how much human beings know about genes!

The road ahead is long!

Chapter 362 Lu Li, the Man Who Leads the Times

Since the gene chip was virtualized, the progress of the experiment has been significantly accelerated.

The detection of gene expression information is similar to making a jigsaw puzzle.It was very difficult at the beginning, and as more and more gene fragments were identified, the speed would become faster and faster.

Seeing the progress of the experiment in the virtual laboratory, Lu Li nodded slightly.According to the current process, the human genome map should be completed within a few years.

Genetic testing continues to be carried out, but gene targeting technology is at a loss.

Before the completion of the human genetic map, it is really difficult to improve gene positioning from the software, that is, the accuracy of information.

Maybe... there is something wrong with my train of thought.

Since the ability of transcriptase is not good, then another ability can do it!

When his thoughts turned to this point, Lu Li suddenly realized.

Transcriptional activators, like effectors (TALEs), are not irreplaceable for gene targeting specificity in human cells!

There must be other undiscovered "enzymes" that could take over the function of transcriptase.

Thus, Lu Li began to study various cells, bacteria and viruses.

Animal cells, plant cells, bacteria, and even viruses, analyze cell structures for enzymes with gene-targeted specificity.

The process was not smooth.

Finding enzymes with target specificity among a wide variety of protein molecules is entirely hit and miss.

Days passed, and enzymes with target specificity remained undiscovered.

Liu Qin has come to Fudan.Just like in reality, Liu Qin became an associate professor at Fudan School of Economics.

It's just... the difference from the reality is that before Liu Qin joined Fudan, he underwent a strict and detailed political review.

Because when Liu Qin was a Ph.D. student at Harvard, his interpersonal relationship was relatively simple and he had no suspicious social relationships.Moreover, Liu's father's business is also based in China, and he has no foreign assets at all, let alone any business dealings with foreign countries.

So... There is no problem with Liu Qin's political trial.

When Liu Qin told Lu Li the news of the political trial, Lu Li was helpless.It turns out that I still need to go through national censorship before I fall in love with someone.

The arrival of Liu Qin enriched Lu Li's weekend time, and he no longer plunged into the laboratory every weekend.

Professor He is very pleased with this.Scientists are human beings and cannot treat themselves as machines.Maybe the coming of love can inspire more inspiration.

These words... turned out to be true!

In the second month after Liu Qin joined Fudan, Lu Li's research finally made a key breakthrough.

He found another enzyme with target specificity - nickase!

Among the type II restriction enzymes, there is also a special type, which only cuts one strand of double-stranded DNA, resulting in a nick. This type of restriction enzyme is a nickase.

From a functional analysis, transcriptase is a large-scale search radar, and nickase is a fire control radar.

In terms of positioning accuracy, the nickase is indeed very precise, almost hitting every target. The problem is... without a large-scale search radar, it is difficult for the fire control radar to find the target.

That being the case...then combine the two.

So, Lu Li started to experiment.

Prepare shear protein, prepare transcriptase, prepare nickase, and then organically combine the three to form a brand new gene editing tool.

Next, Lu Li recorded the guide RNA on the transcriptase to determine the characteristics of the target gene.Then use the gene gun to inject the new gene editing tool into the cells to be edited.

Under the electron microscope, the guide RNA on the transcriptase starts to search for the target on the gene chain, and the guide RNA matches the target gene to initially lock the approximate orientation of the target.

Immediately, the sheared protein begins to move towards the target orientation.

When the cut protein moves to the position of the target, the nicking enzyme fire control radar is turned on, and the accurate target is calibrated instantly, and the target is locked!

In the next moment, the splicing protein moves to the target position and starts to fuse with the gene chain, and the helical gene chain unwinds and flattens.

Then... As if with a "click", the shearing protein accurately cut the marked position.

Good start!The first experiment was successful!

Afterwards, Lu Li repeated the same experiment hundreds of times, and the results of each experiment were all [-]%, without the slightest error!

It worked!

The combination of scanning radar and fire control radar allowed Lu Li to create a brand new gene editing tool.

A revolutionary breakthrough in gene targeting technology!

Since then, human gene editing technology has reached a new level.

The brand-new gene editing tool can accurately lock the target and cut the required gene fragments accurately, no more luck.

Lu Li closed the virtual lab with a bright smile on his face.

Gene technology has made another major breakthrough!

Back in the study, Lu Li sat at the computer desk and began to write his thesis.

"Gene targeting technology, a new gene editing tool."

In the paper, Lu Li described a brand-new gene editing tool with careful logic, rigorous expression and detailed experimental data.

In the original gene editing technology of shearing protein + transcriptase, a nicking enzyme "fire control radar" was creatively added, so that this brand-new gene editing technology has a very high gene targeting accuracy rate, and the gene editing efficiency has become An improvement of tens of thousands of times.

After finishing writing the thesis and printing it out, Lu Li walked out of the office with the materials for the thesis.

"Teacher, I wrote a new paper, please take a look."

Arriving at Professor He's office, Lu Li handed over the paper materials in his hand.

"New thesis? You... have produced results again?"

Professor He secretly sighed, "It's so terrifying", and reached out to take the paper that Lu Li handed over.

"Gene targeting technology? A new gene editing tool?"

Seeing the title of the thesis, Professor He took a breath of air-conditioning, uh... the air-conditioning blowing out!

Lu Li actually came up with a brand new gene editing tool?

Suppressing the shock in his heart, Professor He buried himself in the paper materials in his hand.

After reading it carefully, Professor He has clearly seen from the statement of the paper that a more efficient and more accurate gene editing technology has been officially born.

With this brand new gene editing tool, human gene editing technology has reached a new level.

Lu Li...is really a genius!

First came up with a brand new anti-cancer technology, and now a brand new gene editing tool.

State-owned pillars, the blessing of the country!

Lu Li...will surely lead the future!

Looking up at Lu Li in front of him, Professor He suppressed his excitement and said to Lu Li, "Go! Let's do a test."

"Ok!"

Saying that, the two walked out of the office together and came to the laboratory.

In the laboratory, Lu Li repeated the experimental process in the virtual laboratory again, conducting a hundred gene editing experiments in a row.

100% success rate, 100% accuracy rate.

"It's amazing! It's amazing!"

Professor He picked up the verification data from the laboratory, his hands were shaking, "The efficiency of gene editing has increased by thousands of times. This is a revolutionary breakthrough!"

Lu Li smiled all over his face.

"this essay……"

Professor He looked up at Lu Li, and said seriously, "Delete the specific experimental steps, delete the detailed experimental data, and only make a theoretical statement. Modify it and send it to the top international academic journal 'Nature'."

Then, Professor He looked at the paper in his hand again, and said, "I will submit your current paper to the Chinese Academy of Sciences as an internal document."

"Ok!"

Lu Li agrees with Professor He's approach of "retaining one hand".

Science has no borders, but scientists have nationalities!

We have mastered the latest technological achievements by ourselves, as for others... I will give you a direction, go study it yourself!

Afterwards, Lu Li reworked the thesis and wrote a directional and theoretical thesis.It's just... At the end of the thesis, Lu Li attached the laboratory's verification conclusions.

After submitting his paper to the "Nature" journal, Lu Li posted his paper on the academic website.

……

across the Pacific Ocean.

In the Millikin Institute of Genomics, researchers are working intensely and busy.