Doctor's Life Simulator

Chapter 321 Dean Wen's support, entering the laboratory
At the same time, on the other side of the ocean, an editor of the "Cell" magazine in the United States received a paper submission from Huaguo. After reading it, he found it very interesting. If the ICR mouse inbred line proposed by the author If it is really as good as he introduced, it can completely replace the white mouse in the experimental animal world and become the gold standard in the industry.

This was an epoch-making event. The editor smelled the unusualness, immediately printed out the paper, and walked into the office of the editor-in-chief Alex.

Alex, who is over 60 years old this year, has eye-catching blond hair and a tough facial line that looks like a knife and an axe. His whole person gives off a sense of authority, and he is reviewing some papers very seriously.

Alex is very well-known and influential in the world, and his own resume is also shining. He just retired from the position of technical consultant of Johnson & Johnson and joined "Cell" magazine. He is an extremely prestigious , is also a very courageous editor-in-chief, and also the director of the research institute affiliated to "cell".

Seeing the little editor knocking on the door and coming in, Alex looked up and seemed a little dissatisfied: "Didn't I say that I'm picking the cover article for the next issue these two days, don't come in and disturb me if there's nothing important."

The editor handed over the printout of the thesis in his hand: "Editor-in-Chief, this paper is very interesting, I think you will be interested."

The editor is very familiar with Alex's temper, and he is full of passion and keenness for such groundbreaking papers and research.

Alex was a little surprised. He took the paper from the editor and glanced at the author. It turned out to be a doctor from China. He was a little surprised. Then he opened the content and read it carefully.

After reading it soon, Alex put down the thesis, pondered for a while, then picked it up and read it again.

"The ICR mouse inbred strain surpasses all the inbred strains cultivated in the world in traits. If this can be achieved, it will probably cause a sensation in the animal experiment world."

With Alex's insight, of course, I understand the influence of this paper, which is definitely epoch-making.

More than two-thirds of animal experiments use mice, so an inbred strain of mice with better traits and more stability is enough to make all pharmaceutical companies and scientific research units crazy about it.

The "Cell" journal, in addition to its editorial department, also has a subordinate research institute, which has part-time experts and researchers in various disciplines. It is a top scientific research institution in a multidisciplinary field.

The source of funds for the institute is various pharmaceutical giants such as Pfizer, Johnson & Johnson, and GlaxoSmithKline, which are extremely authoritative internationally.

After reading Qin Lang's thesis, Alex had a crazy idea in his mind, that is, to conduct verification experiments through the research institute affiliated with Cell, as long as the excellent characteristics of the ICR mouse inbred strain are confirmed, then he will immediately start to create animals The new gold standard for testing mouse inbred strains in the experimental field, an authoritative new international standard.

In the future, all mice produced by animal companies must use ICR mice as the standard.

Alex thought for a while and said directly: "You print out ten copies of this paper, and at the same time, notify all the heads of the institute to be present. I'm going to hold a large-scale academic exchange meeting of the institute."

At this time, Qin Lang had also sorted out the first draft of his project.

When I came to Professor Shen Xi's office and saw Qin Lang, Shen Xi was also curious about what new ideas his student had these days?Have you given up the idea of ​​investing in the pharmaceutical field?

"Qin Lang, how is your subject preparation?"

Qin Lang directly handed his first draft to Shen Xi, and said: "Teacher, I have changed my mind. I am going to put down the research and development of new anti-tumor drugs. The traits, stability and superiority evaluation of the ICR mouse inbred line compared with the traditional Kunming white mouse""

"ICR mice?"

Shen Xi was a little at a loss. She was naturally very familiar with the field of animal experiments, but she had never heard of any inbred strain of ICR mice.

"The so-called improved ICR mouse is a new inbred strain that my subject plans to study and improve."

Qin Lang said directly.

"Are you going to improve the experimental mice, and then evaluate and study the traits, stability, and superiority of this mouse?"

Shen Xi was shocked by Qin Lang again, why does this kid think so wildly?

Moreover, I don’t know that your postgraduate tutor, Shen Xi, mainly studies in the field of tumor prevention and treatment, and is also top-notch in the entire Huaguo. How can I play with experimental mice without hugging such an excellent tutor?

"Teacher, in fact, I already have a preliminary theoretical research foundation, and now I need the laboratory to induce the first batch of ICR mice. I have established a cooperation agreement with our Zhejiang Mingshi Pharmaceutical Group, and the other party has provided me with five hundred Ping’s mouse house, as long as the first batch of mice can get out of the laboratory, I will be able to breed a large number of ICR mice in the experimental base of Ming’s Pharmaceutical Group. My project this time is to ask the teachers and students of the whole school to cooperate with me. These inbred strains of ICR mice were scored and evaluated."

"I am willing to provide the college with [-] improved ICR mice for free, and then I will pay for the entire project myself. There is only one requirement, and that is to provide my laboratory with the induction of the first batch of mice."

Qin Lang directly showed his trump card. Rich people are so simple and unpretentious. Taking advantage of the talent, Qin Lang is going to mobilize the teachers and students of the Zijingang No. [-] Academy to work on projects for himself.

To be honest, for the teachers and students of the college, the general project funding is only [-] to [-], and the experimental mice often take up [-] to [-]. If someone can provide mice for free, they are of course very willing.

In itself, this kind of evaluation experiment is very simple to operate, all that is needed is to record the performance of the ICR mice in the experiment.

And the final huge data is sorted out and analyzed by Qin Lang himself.

Hearing Qin Lang's plan, Shen Xi was also a little excited. Qin Lang is too daring to think about it, and he is also very operable.

But the only question is whether this so-called improved ICR mouse is worth the effort to mobilize all the teachers and students of the school for evaluation.

If the inbred strain of ICR mice is not as good as Qin Lang imagined, all follow-up work will be a waste of time.

And this topic will also become a huge laughing stock.

"Qin Lang, like this, I will seek the opinions of other tutors for your project plan, and I need to digest it. It happens that there is still some time left for your opening report, so I will come here first."

Shen Xi waved his hand to let Qin Lang go out first.

Looking at the first draft of the project on the table, Professor Shen muttered to himself: "ICR mouse inbred line? This brat really dares to do and think. It's a headache, and I don't understand the key point!"

The next day, Qin Lang was still sorting out project materials in the dormitory when he received a call from Shen Xi.

"Qin Lang, I have basically talked to Dean Wen Yunqi about your topic and general ideas. Dean Wen expressed his support and should arrange for you to enter the laboratory of the institute in the near future."

In addition to being the dean of Zijingang No. [-] Hospital, Wen Yunqi is also the director of Zijingang Cardiovascular Disease Research Institute and the director of the Heart Center. His resume is even more gorgeous and scary:

Wen Yunqi is the Chief Scientist of the National Major Scientific Research Project (973), the Vice Chairman of the Cardiovascular Branch of the Huaguo Medical Association, and the Chairman of the Cardiovascular Branch of the Zhejiang Medical Association; Co-editor-in-chief of "Chinese Journal of Emergency Medicine", deputy editor-in-chief of "Chinese Journal of Cardiovascular Diseases"; visiting professor at UCLA Reagan Medical Center.

Dean Wen has a very high academic influence in the interventional treatment of heart valve disease and the basic and clinical research of stem cell treatment of cardiac insufficiency.He won 1 first prize of the National Science and Technology Progress Award and 2 first prizes of the Provincial Science and Technology Award as the first completer.

Shen Xi continued to say to Qin Lang: "Qin Lang, Dean Wen still has a good impression of you, and you performed the operation on his aunt, Ms. Wen Xia before. Just take this opportunity, let's meet Dean Wen together. "

Of course, Qin Lang was very happy to meet the boss, Dean Wen. If he could gain the favor and support of Dean Wen, the road ahead would be smooth sailing.

Soon, Shen Xi brought Qin Lang to Dean Wen's office, and saw a thin middle-aged man with gray temples and brown eyes, busy working on the desk, it was Wen Yunqi .

"Dean Wen, hello."

After Qin Lang said hello, Shen Xi arranged to sit beside her.

Dean Wen has a good impression of Qin Lang and is also very curious. After all, he is the younger generation that Wu Jizong values, and he is kind to himself.In the eyes of the directors of various departments in Zijingang No. [-] Hospital, he is also an excellent junior worthy of admiration.

In particular, he dared to raise such a "daring" subject, and he was really a student full of aura.

Although Zijingang University is already one of the top five universities in China, Wen Yunqi feels that sometimes many students are too obedient and lack the spirit of pioneering and adventure. They can become the mainstay of the industry, but they lack the kind of pioneering top students. talent.

Sometimes, Wen Yunqi is also thinking about whether this is the tragedy of Huaguo's education system.

In many ordinary colleges and universities, even some 211 colleges and universities, many students do graduation thesis at the undergraduate or master level, there is no lack of a lot of copy and paste, Chinese translation, and even more outrageous, first translate the article through Youdao Dictionary into English, and then re-translated back to Chinese, with a little adjustment, it was handed in.

As for the research field of the topic, basically, nine out of ten are topics directly fed by the instructor, and very few people propose research topics by themselves.

Therefore, Wen Yunqi's eyes lit up when he saw Qin Lang's research topic and boldly proposed the concept of ICR mouse inbred strain. As for the idea of ​​Qin Lang's teachers and students participating in the school, he appreciated it very much, regardless of the final result of the project No, but this process is very good for cultivating and encouraging students' scientific thinking and bold innovation.

As for Qin Lang's proposed use of the laboratory for tumor prevention and treatment to induce the first batch of mice, it is not a matter of a word for him, the dean:
"Professor Shen, after my consideration of Dr. Qin's topic, there is no problem at all. You can arrange the opening report according to the normal time node, and I will attend it later. At the same time, Dr. Qin entered the laboratory in advance for ICR The induction of mice, I approve."

"Really, that's a big thank you to the dean."

Qin Lang did not expect that Dean Wen agreed so readily.

"Good job."

Wen Yunqi encouraged Qin Lang again, and rushed to attend a meeting.

For this reason, Shen Xi also specially instructed the precautions of the laboratory. As for the other matters of the laboratory's specifications, of course Dr. Wang Rou will help.

With the authorization of Dean Wen and the arrangement of Dean Wen, Qin Lang entered the laboratory the next day.

The laboratories of the First Affiliated Hospital of Zijingang University are equipped with state-of-the-art equipment and various equipment.

At Qin Lang's request, Wu Qingguo was also very cooperative and sent the first batch of conventional Kunming mice needed by Qin Lang to the laboratory.

Although Qin Lang already has the best induction plan through the new drug research and development laboratory, as a real experiment, in order to be convincing and credible, Qin Lang still designed the control group and the experimental group according to the normal process , and established a statistical analysis model.

As for the equipment, Qin Lang also prepared in advance the NTS-1300 constant temperature oscillating water tank from Tokyo Rika Instrument Co., Ltd. and the CX-41 optical microscope from Olympus Corporation through the help of Wang Rou.

As for the reagents, Qin Lang also rigorously configured them according to the test procedures, and made detailed records at the same time. Obviously, this is a very huge workload.

Of course, these reagents and materials were all paid by Qin Lang himself. Looking at the reduced balance, Qin Lang was also moved. Sure enough, doing scientific research is a waste of money. Fortunately, he already has a mature plan, so that Take a detour.

Shen Xi only allowed him to use various instruments in the laboratory.

After all, Qin Lang's project has not yet started. According to Qin Lang's own thinking, there is no project funding. Professor Shen even persuaded Qin Lang to apply for funding first, but he refused.

After all, Qin Lang was willing to give up the money for his big plan, although it was very painful!

Qin Lang suddenly wanted to do some part-time plastic surgery jobs. Speaking of which, he has been busy recently, and occasionally has time to go to Shuguang Meili Plastic Surgery Hospital.

Instead, some particularly high-quality customers were selected.

Qin Lang's current plastic surgery techniques are as pure as fire, double eyelid surgery, and rhinoplasty surgery, all of which have low risks and high returns, especially his basic skills at the textbook level. As long as Qin Lang speaks out, those Internet celebrities are not one by one Came over.

Qin Lang began to prepare the solvent 0.9% sodium chloride injection very carefully, and the positive reagent CP (purity ≥ purity 1) was purchased from Sigma Aldrich.

The preparation concentration was 4mg/m L; fetal calf serum (fetal calf serum, FBS) was purchased from GIBCO company
Potassium chloride (KCl) was purchased from Sigma Aldrich
Use deionized water to prepare 0.075 mol/L. Giemsa dye was purchased from Sigma Aldrich,

以及最重要的临染色前1份Giemsa原液经9份1/15 mol/L磷酸盐缓冲液(1/15 mol/L Na2HPO4溶液和1/15 mol/L KH2PO4溶液1∶1混合，pH6.8)稀释配制为10%Giemsa应用液。

Looking at these reagents purchased from companies all over the world, Qin Lang felt heartbroken again. Fortunately, he had already mastered the final optimal ratio. Otherwise, if he followed the normal experimental procedure to find the optimal ratio, he would have A lot of reagents and time are wasted, and it may not be successful.

After preparing the reagents, Qin Lang began to take out the mice that Wu Qingguo had sorted in advance.

Each batch of Qinlang controls 36 mice. These mice are all male at the age of 7-8 weeks, weighing 30-32g.

Although Qin Lang thinks that these mice are not in the best condition, they are from the animal experiment base of Mingshi Pharmaceutical Group. These mice have passed the ethical review of the Experimental Animal Welfare Ethics Committee of the National Drug Safety Evaluation and Testing Center. .

At the same time, it also has an animal production license, so Qin Lang's experiment is very rigorous and formal in all aspects, and it can be included in the official paper later.

After simulating so many lives, Qin Lang is still very familiar with the basic skills of the experiment.

Qin Lang used TOXSTAT2006 statistical analysis software for random grouping to control individual differences in grouping.

After that, clinical symptom observation and body weight measurement were strictly followed the formal procedures.

Because it is in a real laboratory, Qin Lang can only administer medicines honestly, come once a day in the morning and afternoon, and observe in separate cages, including whether it is dead, dying, activity status, appearance and coat, and whether there is trauma , feces, etc.; the animals were weighed before, during, and before dissection.

In the next week, Qin Lang entered into a very fulfilling experiment.

Although the results are already known, in order to maintain a rigorous database later, Qin Lang still analyzed and recorded the relationship between different dosage concentrations and the types of chromosomal aberrations at the sampling time points.

During this period, Wu Qingguo also came to the laboratory every other day, firstly to deliver mice to Qin Lang, and also to observe Qin Lang's experimental process curiously, and by the way, Qin Lang's recent situation, as well as Qin Lang's recording and dispensing, All kinds of beautiful photos that were breathing and in a daze were sent to Chairman Ming.

Finally on the seventh day, Qin Lang completed the first batch of chromosomally mutagenized ICR mice with stable traits.

Under the arrangement of Qin Lang, Wu Qingguo sent the first batch of male parents to the experimental base and began to breed the first inbred line.

(End of this chapter)