I can extract side effects.
Chapter 624 On how much water a pig can have
Kunming City, Gusu University, Sanqing Branch.
In a well-lit laboratory with three large windows, Huang Qin's fingers were flying and typing on the keyboard rapidly.
Seeing the lines of text flowing out from his fingertips and converging into a research report with detailed data, he raised his head and stretched his waist.
On the long table at the side, there were eight young people with immature faces who were also busy like him.
Some were concentrating on the computer, some were observing carefully under the microscope, and some were sitting together in twos and threes, as if they were having in-depth discussions on the experimental topics.
Huang Qin couldn't help but nodded with satisfaction. Under his influence, the enthusiasm for learning of this group of juniors and juniors has been visibly improved by the naked eye. Everyone has become diligent and hardworking, which really makes him very pleased.
Suddenly, a strong cheer erupted from the corner.
A fat boy jumped up excitedly with his face flushed.
"It's over, it's over, my paper has been accepted."
"I didn't expect that a scumbag like me would publish the core journal of cscd one day."
Another tall, thin boy stood beside him and let out an exclamation.
"Cscd core, tsk tsk, amazing, where did you publish your paper?"
"Biotechnology Bulletin."
"It's the article "Establishment of Pig Six1 and Six2 Gene Knockout Cell Lines", it's amazing my brother."
"Hehe, no matter where, the impact factor is not high, only 0.8." The fat boy scratched his head embarrassingly.
"It's okay, it's the first time we're writing a thesis, so it's great if we can publish it." The tall and thin boy smiled understandingly.
"That's right, I just wrote a random article, I didn't expect it to pass."
"Thanks to the good subject brought by the big brother, otherwise it would be so easy to publish the paper."
While the tall and thin boy was talking, his cell phone rang. When he opened it, it turned out to be a review reply.
He immediately shouted excitedly: "Haha, my thesis has also passed, it is "Progress in Biochemistry and Biophysics"."
The fat boy immediately gave him an admiring look: "Wow, SCI District [-] paper, brother, you are too good."
"I want to learn from you and vote for the second district next time."
The movement of the two immediately attracted the attention of others.
Everyone put down the business at hand one after another, surrounded the two of them, and started talking about it.
Huang Qin also stretched his neck curiously, and looked over there.
He knew in his heart that [-]% of the papers were sent out by everyone, and they all got replies one by one.
These papers were arranged according to his project assignments. Before submitting the papers, his supervisor Du Biao had also done minor revisions. It would definitely be no problem to publish them in the second district or core journals.
After all, it is not a top journal in a district. As long as these students' papers are written without any loopholes, can be justified, and have solid experimental data, there is no reason why they should not be passed after a little summary and analysis of the rules.
The main reason is that the direction of pig organ planting is very cutting-edge, and many fields are still being explored.
As long as there are some original ideas, richer experimental content, and a large number of experimental results to support it, it is a very good paper.
Of course, it is impossible for these students to come up with any breakthrough theories. In his opinion, it is nothing more than doing some trivial genome analysis work, and then writing a thesis to complete the graduation requirements.
Thinking of this, Huang Qin couldn't help clapping his hands, reminding everyone with a smile.
"You all check your mailboxes, maybe there will be surprises."
Only then did everyone suddenly realize that they took out their mobile phones to check their emails.
Sure enough, a scream sounded instantly.
A girl with ponytails waved her mobile phone, a little incoherently excited.
"I've passed it, I've passed it, and it's also a journal in the second district."
"Ah, ah, I'm going to die of excitement."
"Now you can directly graduate with a master's degree."
Immediately afterwards, the others also cheered.
"I have also passed the core journal of cscd, I will make persistent efforts and continue to write one article."
This person read the email, and suddenly became ambitious. He wished he could take the similar experimental data, change a strange angle, and post another article on the spot.
Just for a while, the screams in the laboratory continued to ring out one after another.
The students all received responses from the journals they submitted to.
Without exception, all have passed the review and can be published in major journals.
The lowest level are all cscd core journals, there are about 5 people.
The three with the highest scores were all issued in the second division of SCI.
For a group of Xiao Mengxin who are in the second year of research, this is simply a dream start.
In the first half of the year, they didn't even know how to do the experiment.
Even the gene editing technology was learned in the laboratory with Huang Qin after the project started.
The division of labor between Huang Qin and his mentor Du Biao is very clear. The mentor is responsible for evangelism, employment, and confusion, and they attend meetings everywhere and are familiar with each other.
He is in charge of teaching the brothers and sisters to do experiments, assigning tasks to them, and watching them work.
Of course, such a thing as correcting papers must be done by the supervisor, after all, only he has rich experience in publishing manuscripts in SCI District [-].
Huang Qin, he only has the experience of top-published papers in one district.
The standards of the two are different, and the difficulty is also very different.
Of course, killing chickens doesn't require a sledgehammer, so there is no need for him to revise the papers for these Xiaomengs.
He just needs to do a good job as a contractor while completing his own tasks.
These students are very well-behaved, much more obedient than the employees in his department, they dare not go east if they go west, and they are as docile as little sheep.
Every time Huang Qin taught these rookies to do experiments, he felt like yesterday recurring.
It was as if I had returned to my first day at Sanqing, looking at a large number of instruments in front of me in the laboratory, with a dazed expression and a loss of what to do.
Fortunately, Dawei led him to get started slowly, and did simple experiments one after another, which made him what he is today.
Sometimes I think about it, when Mengxin first started to learn to do experiments, how happy he would be to have a guide who taught him hand in hand.
Maybe because he has been in the rain and had an enthusiastic umbrella holder, so he is not hesitant to be the next umbrella holder.
Anyway, when teaching these juniors and younger sisters to do experiments, he took the trouble to start from the most subtle, and teach all the experience he learned in the work.
He has been doing experiments in Sanqing for so many years, and he has already become familiar with it. He knows the simplest and most effective experimental methods, as well as all precautions.
For him, teaching these students with years of experience is also a kind of enjoyment of being a teacher, and it is also a practice journey of summarizing experience into rules.
In the first two months, the students were still a little bit clueless, but under the encouragement of the project bonus and graduation thesis, they still studied without sleep or food.
With sufficient funding, they were able to repeat the experiments over and over again and make progress.
With the encouragement of their tutor and Huang Qin, the students' confidence has greatly increased, and they feel like a fish in water.
They obtained experimental results from the separating funnel, centrifuge, colorimeter, and PCR sequencing machine in the laboratory, gained unprecedented scientific research experience, and ascended to a noble, rational, and noble academic kingdom.
In just three months, Huang Qin has seen that there are several good seedlings suitable for scientific research.
And all of this, finally today, has borne rich fruits.
In a short period of time, everyone got the result.
Huang Qin also saw their list of papers on the computer.
"Establishment of Pax2 Gene Knockout Pig Cell Line"
"Molecular information analysis of porcine SALL1 protein and preparation of knockout cell lines"
"SCD Gene Knockout Pig Cell Line and Its Construction Method"
"Construction of Porcine Vascular Endothelial Cell Surface Antigen Gene Knockout Cell Line"
"A method for the construction of a gene-modified cell surface Fas molecular pig model"
"Comparison of homology of human/pig complement regulatory proteins and establishment of porcine GGTA1 gene transfected cell line"
"Establishment of Porcine Six1 and Six2 Gene Knockout Cell Lines"
"Establishment of pig cell line transfected with humanized TFPI blood coagulation regulatory gene"
Eight papers, all of which are related to pigs.
Either insert a foreign gene in the pig cell genome, or knock out the related endogenous gene.
In this way, knocking out specific antigen genes and transferring humanized genes can not only effectively regulate immunological incompatibility genes, but also improve coagulation dysfunction, thereby effectively inhibiting the occurrence of transplant rejection.
However, there are only eight papers, which is far from enough.
These immature students can squeeze more achievements from them.
The cleaner it is squeezed, the richer it will be.
There are more than 30 immune and blood coagulation genes in total. It is necessary to improve all the genes to figure out the relationship between them and find out the most critical genes.
At least 20 more papers can be published from these studies, and each person has three or four papers, which is enough for them to be busy.
Not only must people be squeezed clean, but also the most fat and water must be squeezed out of pigs.
Thinking of this, Huang Qin's eyes brightened, and he said with a smile.
"Everyone's research over the past few months seems to have yielded a lot."
"Eight papers, everyone did not fall behind, really gratifying."
"I don't know if you still remember a sentence I once said."
"What are you talking about?" Everyone fell silent, their ears pricked up, and they looked over in unison.
"I told you a long time ago that every time you publish a paper, you will get a bonus of 2 yuan. Have you all forgotten? It shouldn't be."
Huang Qin laughed out loud, looking incredible.
Students, you looked at me, I looked at you, and immediately laughed too.
"Eldest brother, how could you forget this, I'm waiting for you to talk about it yourself." The fat boy joked.
"You are our food and clothing parent. Everyone respectfully calls you a big brother, and no one dares to urge you."
Huang Qin chuckled, "It's still my fault. Come on, go through the process quickly and submit the application to me. If you hurry up, the money should arrive at the end of this month."
Everyone was overjoyed immediately, and all of them smiled with open eyes, smiling so hard that they couldn't see their teeth.
"Wow, that's really great. I'll make a fortune by the end of the month."
Everyone thought that in addition to the living allowance of 5000, there is also a bonus of 2 yuan for thesis. By the end of this month, they will get [-] yuan, and they suddenly feel like a pie in the sky.
I can't wait to pinch my thigh immediately to see if I am dreaming.
Looking at this scene, Huang Qin was very satisfied, and asked with a smile.
"Isn't it cool to post a paper on the second district?"
"Cool!" Everyone couldn't help but nodded.
"Wouldn't it be nice to get 2 yuan in the account after publishing the thesis?" Huang Qin followed suit.
"It's cool, it's so cool!" Everyone shouted out loud.
"Then do you want to publish more papers and earn a few more 2 yuan?" Huang Qin finally wanted to see.
Everyone said in unison: "Of course."
"Eldest brother, don't pamper me just because I'm Jiaohua, use money to torture me severely."
"Don't stop!"
"A lot of money, and a lot of papers. Who doesn't want such a good life."
"That's right, if there are any experiments, let them come here, and come to me!"
Everyone has published papers and has money in their pockets, and they are all confident.
Huang Qin couldn't help showing a smug smile like an old fox: "I'm just waiting for you."
After swiping a few times, he mass-sent an experiment list directory to everyone.
"There are more than 30 immune-related pig genes that we need to study, and some of them have already been done. I have discussed other experiments with my supervisor, and they are in this list."
"You pick one by one slowly, find the one you are interested in, at least 3-4 per person, then mark it down, and start a new experiment tomorrow."
"That's about it. Everyone shouted so enthusiastically just now, I hope I won't be disappointed."
Everyone couldn't wait to open the file, and when they saw it, they gasped.
"Hiss, there are so many?"
"I never thought that a pig could write so many papers!"
"Damn, how long have you been preparing for this, big brother?"
"If it weren't for our large number of people, I'm afraid we would have to go through the year of the monkey."
"It's not just 996, it's 007."
"Although there are many experiments, there is also a lot of money. If I choose 4 experiments, I can publish 4 papers. Including which one I just published, darling, the bonus alone is 10 yuan."
Now, everyone is not calm.
Soon, within 5 minutes, everyone enthusiastically divided up the experiments.
Everyone was assigned 4 experiments, and none of them fell.
Huang Qin waved his hand very satisfied: "Students, next, it's time to explode."
"Don't worry, I will definitely stand with you and explode your liver with you."
"From today, I will eat, live and work with you all together."
"We must figure out the role of all immune-related genes as soon as possible, and then make the cleanest pig blastocyst. After it matures, the organs on its body will be transplanted to baboons for further clinical verification."
"If the feasibility of gene-knockout pig-baboon xenotransplantation is confirmed, it will be the biggest breakthrough in the field of organ transplantation in recent years."
"Foreign scientists have done similar experiments, transplanting non-genetically modified pig livers to non-human primates. As a result, after the transplantation, heterogeneous immune rejection occurred immediately, and the survival time did not exceed 12 hours."
"Later, scientists transplanted GGTA1-knockout pig livers into baboons, but they only survived for 5 days."
"While the gene knockout extended the survival of transplanted organs, other immune rejection reactions persisted, along with more severe coagulation disturbances."
"So, we have to get that out of the way and move on to that."
"At least the xenograft organs must survive in the baboons for more than 3 months in good health, and there are no complications to prove our success."
"I am very much looking forward to waiting with you for this day to come."
In a well-lit laboratory with three large windows, Huang Qin's fingers were flying and typing on the keyboard rapidly.
Seeing the lines of text flowing out from his fingertips and converging into a research report with detailed data, he raised his head and stretched his waist.
On the long table at the side, there were eight young people with immature faces who were also busy like him.
Some were concentrating on the computer, some were observing carefully under the microscope, and some were sitting together in twos and threes, as if they were having in-depth discussions on the experimental topics.
Huang Qin couldn't help but nodded with satisfaction. Under his influence, the enthusiasm for learning of this group of juniors and juniors has been visibly improved by the naked eye. Everyone has become diligent and hardworking, which really makes him very pleased.
Suddenly, a strong cheer erupted from the corner.
A fat boy jumped up excitedly with his face flushed.
"It's over, it's over, my paper has been accepted."
"I didn't expect that a scumbag like me would publish the core journal of cscd one day."
Another tall, thin boy stood beside him and let out an exclamation.
"Cscd core, tsk tsk, amazing, where did you publish your paper?"
"Biotechnology Bulletin."
"It's the article "Establishment of Pig Six1 and Six2 Gene Knockout Cell Lines", it's amazing my brother."
"Hehe, no matter where, the impact factor is not high, only 0.8." The fat boy scratched his head embarrassingly.
"It's okay, it's the first time we're writing a thesis, so it's great if we can publish it." The tall and thin boy smiled understandingly.
"That's right, I just wrote a random article, I didn't expect it to pass."
"Thanks to the good subject brought by the big brother, otherwise it would be so easy to publish the paper."
While the tall and thin boy was talking, his cell phone rang. When he opened it, it turned out to be a review reply.
He immediately shouted excitedly: "Haha, my thesis has also passed, it is "Progress in Biochemistry and Biophysics"."
The fat boy immediately gave him an admiring look: "Wow, SCI District [-] paper, brother, you are too good."
"I want to learn from you and vote for the second district next time."
The movement of the two immediately attracted the attention of others.
Everyone put down the business at hand one after another, surrounded the two of them, and started talking about it.
Huang Qin also stretched his neck curiously, and looked over there.
He knew in his heart that [-]% of the papers were sent out by everyone, and they all got replies one by one.
These papers were arranged according to his project assignments. Before submitting the papers, his supervisor Du Biao had also done minor revisions. It would definitely be no problem to publish them in the second district or core journals.
After all, it is not a top journal in a district. As long as these students' papers are written without any loopholes, can be justified, and have solid experimental data, there is no reason why they should not be passed after a little summary and analysis of the rules.
The main reason is that the direction of pig organ planting is very cutting-edge, and many fields are still being explored.
As long as there are some original ideas, richer experimental content, and a large number of experimental results to support it, it is a very good paper.
Of course, it is impossible for these students to come up with any breakthrough theories. In his opinion, it is nothing more than doing some trivial genome analysis work, and then writing a thesis to complete the graduation requirements.
Thinking of this, Huang Qin couldn't help clapping his hands, reminding everyone with a smile.
"You all check your mailboxes, maybe there will be surprises."
Only then did everyone suddenly realize that they took out their mobile phones to check their emails.
Sure enough, a scream sounded instantly.
A girl with ponytails waved her mobile phone, a little incoherently excited.
"I've passed it, I've passed it, and it's also a journal in the second district."
"Ah, ah, I'm going to die of excitement."
"Now you can directly graduate with a master's degree."
Immediately afterwards, the others also cheered.
"I have also passed the core journal of cscd, I will make persistent efforts and continue to write one article."
This person read the email, and suddenly became ambitious. He wished he could take the similar experimental data, change a strange angle, and post another article on the spot.
Just for a while, the screams in the laboratory continued to ring out one after another.
The students all received responses from the journals they submitted to.
Without exception, all have passed the review and can be published in major journals.
The lowest level are all cscd core journals, there are about 5 people.
The three with the highest scores were all issued in the second division of SCI.
For a group of Xiao Mengxin who are in the second year of research, this is simply a dream start.
In the first half of the year, they didn't even know how to do the experiment.
Even the gene editing technology was learned in the laboratory with Huang Qin after the project started.
The division of labor between Huang Qin and his mentor Du Biao is very clear. The mentor is responsible for evangelism, employment, and confusion, and they attend meetings everywhere and are familiar with each other.
He is in charge of teaching the brothers and sisters to do experiments, assigning tasks to them, and watching them work.
Of course, such a thing as correcting papers must be done by the supervisor, after all, only he has rich experience in publishing manuscripts in SCI District [-].
Huang Qin, he only has the experience of top-published papers in one district.
The standards of the two are different, and the difficulty is also very different.
Of course, killing chickens doesn't require a sledgehammer, so there is no need for him to revise the papers for these Xiaomengs.
He just needs to do a good job as a contractor while completing his own tasks.
These students are very well-behaved, much more obedient than the employees in his department, they dare not go east if they go west, and they are as docile as little sheep.
Every time Huang Qin taught these rookies to do experiments, he felt like yesterday recurring.
It was as if I had returned to my first day at Sanqing, looking at a large number of instruments in front of me in the laboratory, with a dazed expression and a loss of what to do.
Fortunately, Dawei led him to get started slowly, and did simple experiments one after another, which made him what he is today.
Sometimes I think about it, when Mengxin first started to learn to do experiments, how happy he would be to have a guide who taught him hand in hand.
Maybe because he has been in the rain and had an enthusiastic umbrella holder, so he is not hesitant to be the next umbrella holder.
Anyway, when teaching these juniors and younger sisters to do experiments, he took the trouble to start from the most subtle, and teach all the experience he learned in the work.
He has been doing experiments in Sanqing for so many years, and he has already become familiar with it. He knows the simplest and most effective experimental methods, as well as all precautions.
For him, teaching these students with years of experience is also a kind of enjoyment of being a teacher, and it is also a practice journey of summarizing experience into rules.
In the first two months, the students were still a little bit clueless, but under the encouragement of the project bonus and graduation thesis, they still studied without sleep or food.
With sufficient funding, they were able to repeat the experiments over and over again and make progress.
With the encouragement of their tutor and Huang Qin, the students' confidence has greatly increased, and they feel like a fish in water.
They obtained experimental results from the separating funnel, centrifuge, colorimeter, and PCR sequencing machine in the laboratory, gained unprecedented scientific research experience, and ascended to a noble, rational, and noble academic kingdom.
In just three months, Huang Qin has seen that there are several good seedlings suitable for scientific research.
And all of this, finally today, has borne rich fruits.
In a short period of time, everyone got the result.
Huang Qin also saw their list of papers on the computer.
"Establishment of Pax2 Gene Knockout Pig Cell Line"
"Molecular information analysis of porcine SALL1 protein and preparation of knockout cell lines"
"SCD Gene Knockout Pig Cell Line and Its Construction Method"
"Construction of Porcine Vascular Endothelial Cell Surface Antigen Gene Knockout Cell Line"
"A method for the construction of a gene-modified cell surface Fas molecular pig model"
"Comparison of homology of human/pig complement regulatory proteins and establishment of porcine GGTA1 gene transfected cell line"
"Establishment of Porcine Six1 and Six2 Gene Knockout Cell Lines"
"Establishment of pig cell line transfected with humanized TFPI blood coagulation regulatory gene"
Eight papers, all of which are related to pigs.
Either insert a foreign gene in the pig cell genome, or knock out the related endogenous gene.
In this way, knocking out specific antigen genes and transferring humanized genes can not only effectively regulate immunological incompatibility genes, but also improve coagulation dysfunction, thereby effectively inhibiting the occurrence of transplant rejection.
However, there are only eight papers, which is far from enough.
These immature students can squeeze more achievements from them.
The cleaner it is squeezed, the richer it will be.
There are more than 30 immune and blood coagulation genes in total. It is necessary to improve all the genes to figure out the relationship between them and find out the most critical genes.
At least 20 more papers can be published from these studies, and each person has three or four papers, which is enough for them to be busy.
Not only must people be squeezed clean, but also the most fat and water must be squeezed out of pigs.
Thinking of this, Huang Qin's eyes brightened, and he said with a smile.
"Everyone's research over the past few months seems to have yielded a lot."
"Eight papers, everyone did not fall behind, really gratifying."
"I don't know if you still remember a sentence I once said."
"What are you talking about?" Everyone fell silent, their ears pricked up, and they looked over in unison.
"I told you a long time ago that every time you publish a paper, you will get a bonus of 2 yuan. Have you all forgotten? It shouldn't be."
Huang Qin laughed out loud, looking incredible.
Students, you looked at me, I looked at you, and immediately laughed too.
"Eldest brother, how could you forget this, I'm waiting for you to talk about it yourself." The fat boy joked.
"You are our food and clothing parent. Everyone respectfully calls you a big brother, and no one dares to urge you."
Huang Qin chuckled, "It's still my fault. Come on, go through the process quickly and submit the application to me. If you hurry up, the money should arrive at the end of this month."
Everyone was overjoyed immediately, and all of them smiled with open eyes, smiling so hard that they couldn't see their teeth.
"Wow, that's really great. I'll make a fortune by the end of the month."
Everyone thought that in addition to the living allowance of 5000, there is also a bonus of 2 yuan for thesis. By the end of this month, they will get [-] yuan, and they suddenly feel like a pie in the sky.
I can't wait to pinch my thigh immediately to see if I am dreaming.
Looking at this scene, Huang Qin was very satisfied, and asked with a smile.
"Isn't it cool to post a paper on the second district?"
"Cool!" Everyone couldn't help but nodded.
"Wouldn't it be nice to get 2 yuan in the account after publishing the thesis?" Huang Qin followed suit.
"It's cool, it's so cool!" Everyone shouted out loud.
"Then do you want to publish more papers and earn a few more 2 yuan?" Huang Qin finally wanted to see.
Everyone said in unison: "Of course."
"Eldest brother, don't pamper me just because I'm Jiaohua, use money to torture me severely."
"Don't stop!"
"A lot of money, and a lot of papers. Who doesn't want such a good life."
"That's right, if there are any experiments, let them come here, and come to me!"
Everyone has published papers and has money in their pockets, and they are all confident.
Huang Qin couldn't help showing a smug smile like an old fox: "I'm just waiting for you."
After swiping a few times, he mass-sent an experiment list directory to everyone.
"There are more than 30 immune-related pig genes that we need to study, and some of them have already been done. I have discussed other experiments with my supervisor, and they are in this list."
"You pick one by one slowly, find the one you are interested in, at least 3-4 per person, then mark it down, and start a new experiment tomorrow."
"That's about it. Everyone shouted so enthusiastically just now, I hope I won't be disappointed."
Everyone couldn't wait to open the file, and when they saw it, they gasped.
"Hiss, there are so many?"
"I never thought that a pig could write so many papers!"
"Damn, how long have you been preparing for this, big brother?"
"If it weren't for our large number of people, I'm afraid we would have to go through the year of the monkey."
"It's not just 996, it's 007."
"Although there are many experiments, there is also a lot of money. If I choose 4 experiments, I can publish 4 papers. Including which one I just published, darling, the bonus alone is 10 yuan."
Now, everyone is not calm.
Soon, within 5 minutes, everyone enthusiastically divided up the experiments.
Everyone was assigned 4 experiments, and none of them fell.
Huang Qin waved his hand very satisfied: "Students, next, it's time to explode."
"Don't worry, I will definitely stand with you and explode your liver with you."
"From today, I will eat, live and work with you all together."
"We must figure out the role of all immune-related genes as soon as possible, and then make the cleanest pig blastocyst. After it matures, the organs on its body will be transplanted to baboons for further clinical verification."
"If the feasibility of gene-knockout pig-baboon xenotransplantation is confirmed, it will be the biggest breakthrough in the field of organ transplantation in recent years."
"Foreign scientists have done similar experiments, transplanting non-genetically modified pig livers to non-human primates. As a result, after the transplantation, heterogeneous immune rejection occurred immediately, and the survival time did not exceed 12 hours."
"Later, scientists transplanted GGTA1-knockout pig livers into baboons, but they only survived for 5 days."
"While the gene knockout extended the survival of transplanted organs, other immune rejection reactions persisted, along with more severe coagulation disturbances."
"So, we have to get that out of the way and move on to that."
"At least the xenograft organs must survive in the baboons for more than 3 months in good health, and there are no complications to prove our success."
"I am very much looking forward to waiting with you for this day to come."
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