I can extract side effects.

Chapter 207 The advantage is in me
There are two circles of people in the laboratory, one left and one right, noisy and quiet.

The people in the circle on the right are silently sequencing, with serious attitudes and orderly actions.

There are two new sequencers made by Daxia Zhizhi. Although the working principle is different from that of Immana, they are both second-generation sequencing technology. Everyone is familiar with it, so it is not too difficult to operate.

They are now testing the samples of the gene bank with a new machine to familiarize themselves with the operation of the platform, analyze the data, and then compare the data of different platforms.

It takes a long time for the second-generation sequencer to produce the results, and it takes several days. They have done the test and just wait for the results.

The circle on the right was very lively, people gathered in a circle, discussing a lot.

There are three small and exquisite palm-sized sequencers on the operating table inside, and three employees are carefully operating them.

Standing next to the three of them was the gentle Jiang Kai, who had also been promoted and became the person in charge of the genetic laboratory.

At this moment, he was smiling all over his face, staring at the three instruments with bright eyes, and was assigning tasks.

"A new toy is here today. Don't worry, everyone. Come here one by one. Everyone has a chance. You can first select the gene sequence you want to test, and then register there."

"Everyone knows that the nanopore sequencer has the characteristics of small size, fast library construction, and real-time data generation. Compared with existing sequencing technologies, one of its key advantages is longer read length, which is significant in human genome sequencing. advantage, making it easier to detect large structural changes in a single genome."

"The more fun things are, the more we have to stay behind, from easy to difficult. So today we start with bacteria and viruses, then we start testing mice, and finally we test human DNA sequences."

"Someone is already testing E. coli, yeast, and adenovirus. You have to think of some new varieties next, and you can't repeat the test."

"After everyone has finished the test, they need to analyze the data, compare it with the known data, and submit a report."

The crowd of onlookers cheered, all eager to try.

Huang Qin glanced at the sequencing targets of other colleagues, and found that there were all kinds of targets, ranging from bacteria, viruses, to animal and human DNA.

He registered his own DNA. He was curious about the ability of nanopore technology to test long-sequence genomes. He had already tested his own genome and knew the data by heart. Naturally, he was the best control sample.

Williams made the same choice as him.

After Huang Qin registered, he squeezed to the side of his colleagues who were operating and watched carefully.

I saw this colleague carefully put the E. coli kit into the machine, connect it to his laptop, and wait for the real-time results.

At the heart of the machine, an enzyme unwinds the double strands of DNA and passes one strand through the protein pore, where the unique shape of each DNA base causes a characteristic electrical disturbance to read the sequence.

During the whole process, the long DNA chain is like a long line of people at the subway ticket gate, and each base is like a passenger, entering a narrow nanopore, swiping inside, the current flashes, and the identity is verified, and then The next one continues to pass.

Soon, real-time readouts of DNA fragments are displayed on computer software.

The operator exclaimed while looking at it: "It's incredible, it's really fast and accurate, and the agreement with the previous test data has reached 98%. Although the accuracy is still a little bit worse, it is still no problem in actual use. .”

Jiang Kai stepped forward quickly, leaned in front of the computer screen, scanned it quickly, and nodded approvingly.

"This thing is really good. In the future, we will be able to test as much as we want by sequencing, and we don't have to wait until we have a certain number."

"The Escherichia coli you tested is drug-resistant, right? Look here, there are a large number of structural variations in its genome, which cannot be detected by the data of the next-generation sequencer. Now it seems obvious. To us Studying its resistance mechanism is very helpful."

"This detection technology can realize real-time output of data and real-time analysis, which is really suitable for rapid detection of microorganisms."

Suddenly, warm applause sounded in the laboratory, accompanied by bursts of whispers.

"This is the progress of domestic technology!"

"I didn't expect domestic sequencers to be so advanced."

"I've only heard of this stuff being available abroad, but I didn't expect it to be made in China now."

"Looking at it this way, it's no worse than foreign equipment at all."

"It's so convenient, I don't have to wait a few days for the results anymore."

Everyone had excited expressions on their faces, and they were still discussing endlessly when they returned to the office.

Huang Qin was also shocked. He looked at Williams who was stunned beside him, and felt a flash of joy inexplicably.

"Dawei, have you used a nanopore sequencer before? Is there a big gap with this machine?"

Williams looked shocked, and the exclamation in his mouth couldn't help popping out: "Amazing, unbelievable, this turned out to be a machine made in China. I used a machine from Oxford Nanopore before, and that was a few years ago. Yes, the machine is still very crude, it’s incomparable with this one.”

"However, their new machine is very mini, only the size of a chocolate bar, and its performance has been greatly improved. It should be comparable to this one."

Huang Qin nodded reservedly, and looked at the sequencer made by Daxia Zhizhi: "The fourth-generation sequencing technology seems to be promising for us to overtake, but I don't know if the second-generation machine can compare to Immena."

"Yeah," Williams looked over, a little worried: "If the second-generation sequencer is no different from Illumina, then Illumina will be in danger. There will be a big change in the global sequencing field. "

Three days later, the sequencing results of Daxia Zhizao came out.

Jiang Kai looked at the analysis data in his hand, frowned, and then relaxed it soon.

"Compared with Illumina, there is no difference in the read length among the mainstream dosage forms, and they are all fragments of 50-150."

"There are some gaps in the quality of the obtained data. Immina's is more accurate and can reach 99.9%, and Daxia Zhizao's is between 97 and 99%. The data is unstable and may need to be tested several times. But this is originally a splicing gene. Fragment, it should be technically resolvable."

"Overall, the accuracy of the test data is not bad, but if the algorithm can be optimized, the accuracy will be higher."

"The data analysis is a bit of a stretch, and there is a big gap with Immena, and it is not as easy to use as our own software."

"The test results are usable, but we need to optimize the algorithm and use our own data analysis software."

The analysis results were reported quickly and received affirmative approval.

******
In the dead of night, Williams tossed and turned, tossing and turning, unable to fall asleep for a long time.

Finally, he gets up and takes an old laptop from the suitcase under the bed.

After some secrecy, an email was sent to an address that looked like gibberish.

"This move sucks."

"After the localization of the entire line of Sanqing, we are the ones who suffer."

After 5 minutes, there was an immediate reply.

"Take care of yourself."

"Don't teach me how to do things, Sanqing is just a special case, there will be no second one."

"The localization of the whole line is not so easy."

"Overall, the advantage is with me."

The corner of Williams' mouth twitched for a while, and he was speechless for a while, and an unknown fire rose in his heart.

So arrogant!

The gentlemen on the other side of the ocean have been in the office for a long time, and the space they live in has been folded with reality.

They couldn't see Sanqing's true strength, unlike him, they were in it, and they knew exactly how terrifying Sanqing was.

That's all, he's just a worker.

Why bother so much, just do your own thing well.

After all, this is also the instruction above.

(End of this chapter)