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Chapter 948 Experiment (On the third day of appendicitis, please continue to subscribe~)

Passing the torch from generation to generation is not just as simple as lighting a new firewood from the remaining fire.In the next two months, Dr. Pascal tried his best to sum up the experience and lessons of administrative work, and then pass them on to Sun Lien - he had to choose a time for his review and experiment, so that Sun Lien would have time to accept the "inheritance" ".

All in all, inheritance is not easy, Lao Pa sighed.

On the other hand, Sun Lien finally began to lose his hair in the third year of entering the medical industry.

Every morning when he wakes up... Sun Lien doesn't notice the pile of fresh hair loss on his pillow.After all, he didn't have time to look back at the pillowcase.Basically, it will take a day or two before Sun Li'en finds a dense patch of short hair on his pillow.

It looks like you went to the barber shop and went to bed without washing your hair.

Even Hu Jia would occasionally remind Sun Lien worriedly, "Your hair looks not as thick as before..."

However, such torture is about to see the first ray of victory.On the second Saturday in March, Sun Lien, who had worked hard for two months, left the exam room with a sense of relief after a three-hour exam.

There are not a few medical masters who also took the exam, but almost all of them are older than Sun Lien.After all, this test is a mandatory requirement for applying for an on-the-job doctorate, and the full-time doctoral degree has different requirements for this test-some schools do not require this score.

Taking the exam with a group of seniors who looked almost the same age as Zhou Jun, this feeling is quite... unique.

After coming out of the examination room, Sun Lien drove directly to the laboratory.Before entering the examination room, Shen Xi sent herself a WeChat message, saying that the preliminary experimental results had been obtained.Please Sun Li'en go over and take a look at the data as soon as possible.

From January to March, the actual working time left for Shen Xi is only more than 50 days.During these fifty days, he alone completed the experiment on 240 AQP4 knockout mice in the laboratory.

Divide 240 little mice into three groups, establish control group and experimental group respectively.The control group was treated with diuretics and mannitol respectively.In the experimental group, different drugs were used as initiators of AQP4 protein phosphorylation, and then these mice exhibiting cytotoxic brain edema were treated.And compare the treatment status of these mice after treatment.

The experiment sounds simple, but the actual operation is complicated and time-consuming to the point of outrageous.Intravenously inject drugs to the mice, fix the mice and send them to MRI for inspection and monitoring, give the mice drugs and observe the termination of treatment, and send the number of mice after the experiment to different breeding rooms for observation.Then the mice were killed at the five time points of 24 hours, 72 hours, seven days, fifteen days, and 30 days after the treatment was completed, and their brain tissues were extracted for pathological analysis, and the brain edema of the mice was judged and analyzed. Improved.

A small white mouse would cost Shen Xi a lot of energy and time to perfect the experimental content.And here are 240 mice.

Shen Xi basically tried all the methods that could improve efficiency, but the final effective method was to put ten mice from the same group into the MRI for imaging examination.

If it wasn't for the final rest, there was really no way, so he spent Sun Lien's experimental funds to go to an outside "scientific research company" to hire six staff with a master's degree or even a doctorate degree with rich experimental experience. If they come to the laboratory as experimenters, This experiment wants to complete all data collection and analysis on the second Saturday in March...that is simply an impossible task.

"Awesome." After seeing Shen Xi with deep and heavy dark circles in the laboratory, Sun Lien didn't even look at his own experimental data first, so he tried to get Shen Xi to go back and take a good rest.After being repeatedly rejected by Shen Xi, Sun Li'en only looked at the data.It has to be said that Shen Xi is really good at conducting experiments.Not only the experimental data are detailed, the pictures are clear and easy to read, but also the logical relationship and proportion of the experimental results and speculative evidence are well balanced-the data effectively proves the feasibility of the treatment plan proposed by Sun Lien, and proves that Sun Lien's treatment plan is The best of all the solutions so far.

"Although your plan is a bit troublesome, it is really easy to use." Shen Xi poured coffee into her mouth while commenting on Sun Lien's experimental plan, "The dehydration effect is obvious, and there is no rebound. At the same time, the drug's The safety is also enough - 25% of the mice that use phorbol as the starter died on the spot, this Russian roulette-like treatment plan is actually not even meaningful for comparison..."

"Whether it's significant or not, I don't think we can discuss it for the time being. Anyway, these data are definitely enough to improve the paper." Sun Lien first expressed positive affirmation of Shen Xi's work results, and then asked tentatively, "I heard that , Your boss has some... comments on you recently?"

There are ghosts without opinions.Even Sun Li'en, who is as ill-informed as the news, knows that Shen Xi's mentor has quarreled with Liu Tangchun several times in recent days, and even threatened to "bring the lawsuit to the academic committee."The postgraduates I have recruited and trained through hard work are dedicated to helping others with voluntary experiments, and they have been there for several months-any tutor would be angry.

"I can handle this." Shen Xi coughed, and then said with a smile, "It's just a two-month delay in the experiment, I'll just make it up later."

If it could be done so easily, why is your boss arguing with Liu Tangchun?Sun Li'en gave a wry smile, and then said seriously, "Let me tell you the truth... You know me too, I'm just a clinical candidate. Let me do scientific research. I'm not good at it, and I can't actually do it well either."

Shen Xi nodded in confusion, he didn't quite understand what Sun Lien meant by that.

"However, the treatment and tasks given to me by the academy are inseparable from scientific research. If these exceptional honors don't produce enough weighty results later, it will be like setting me on fire..." Sun Lien sighed He took a deep breath, and then continued, "So, I would like to ask you for a favor—how about working in my laboratory in the future? Not as an experimental assistant who came to help, but as an important member of the laboratory, managing this experiment The daily routine and work of the office, you know, I'm not very good at it."

Perhaps because he was worried about not having enough weight, Sun Lien added, "In the future, as long as I have a paper here, I will definitely have yours—as long as it is available in the laboratory and can be given, you can give it as much as you want. "

　Tomorrow review, I hope it can be successfully converted to oral treatment.

　　I personally feel pretty good, basically no abdominal pain problem.It's just that the diarrhea has been a little serious recently, maybe it's a side effect of antibiotics?

　　
　
(End of this chapter)